Set on 14 acres within Cleveland Park, the Greenville Zoo houses more than 350 animals in recreated terrestrial habitats. The walking trails typically take between 60 and 90 minutes to tour, guiding guests to creatures such as lions, orangutans, and elephants. Giant tortoises lumber along to the delight of kids watching, and just down the path, giraffes curl their black tongues around clusters of leaves.

Through their accreditation from the Association of Zoos and Aquariums, zookeepers uphold high standards in animal care, from maintaining realistic habitats to ensuring animals eat what they would in the wild. These AZA standards extend through other parts of the zoo's operations as well, including its educational programming and conservation efforts—the Quarters for Conservation initiative raises funds for local and global conservation programs.

To ensure that visits are relaxing and convenient, the zoo grounds include two playgrounds and several picnic facilities. The surrounding Cleveland Park affords further recreation such as walking paths and tennis courts.